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KMID : 0903519850280020098
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1985 Volume.28 No. 2 p.98 ~ p.105
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Isolation and Identification of Cellulomonas fimi , Characteristics of its Cellulase and Conversion of the Sawdust into Ethanol
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Abstract
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In the sheep and cattle´s rumen, facultative anaerobic cellulolytic bacteria were isolated by using Hungate´s roll tube technique. In the 21 isolated species, one was screened by its strong cellulolytic activity and identified as Cellulomonas fimi C-14 by investigate morphological, cultural, physiological characteristics and electron microgram.
Optimum conditions of the cell growth and enzyme production were pH 6.5 and 30℃, Thiamine and biotin support a good growth of C. fimi C-14. In the enzyme activities, Crystalline cellulose hydrolyzing activity, CMCase activity and β-glucosidase activity were 20.6, 226.6 and 0.56(unit×10³/㎖) at pH 6.0, 40℃.
By addition of fungal cellulase, enzyme activity was increased. Simultaneous Saccharification Fermentation is better than two step fermentation in ethanol yield with Saccharomyces cerevisiae DY 2.
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